Προβολή Εργασίας
MICRO-SCALE PROCESSING AND DISSOCIATION OF EMBRYOID BODIES DURING LAMINAR FLOW IN A CAPILLARY SYSTEM
Ι. Παπαντωνίου, F. Veraitch, M. Hoare
Department of Biochemical Engineering, University College London |
ΣΥΝΤΟΜΗ ΠΕΡΙΛΗΨΗ
The in-vitro differentiation of mouse embryonic stem cells has as a result the expansion of the cells and the formation of spherical embryoid bodies (EBs) which under the effect of retinoic acid will contain neural precursor cells. In order to produce differentiated embryonic stem cells for regenerative medicine applications there is need to process and dissociate EBs in a consistent manner. The shift to automated process operations in biological systems in the future will eventually have to substitute the ongoing manual methods guaranteeing a reproducible efficacy and safety.
In this work mouse embryonic stem cells were cultured and differentiated into the formation of EBs. On day 8 of their growth the EBs were processed in a well characterised flow environment such as laminar flow within a capillary system in the absence of trypsin. The gradual decrease of size of the EBs and the viability of the released cells under diverse flow conditions were the parameters that were monitored throughout the experimental procedure.
Λέξεις Κλειδιά
Neural precursor stem cell, Embryoid body, Capillary, Shear rate, Residence time, Size distribution, Viability
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